The Rutgers Scholar, electronic journal, Undergraduate Education, undergraduate research
The Rutgers Scholar, electronic journal, Undergraduate Education, undergraduate research

Mercury resistance and merA sequences of moderately
thermophilic and mesophilic bacteria
from hydrothermal vents

Y. S. Chew1, C. Vetriani2, and T. Barkay3
1Cook College,
Rutgers University, New Brunswick New Jersey 08901

2Institute of Marine & Coastal Science,
Rutgers University, New Brunswick New Jersey 08901

3Department of Biochemistry & Microbiology,
Rutgers University, New Brunswick New Jersey 08901



Table 2: Hydrothermal vent isolates, their Hg(II) resistance level and Hg(II) volatilization activity.


Optimum

Growth

Temperature

Isolates

Sample

Site

Genus of

Closest Relative1

Growth in the presence of Hg(II)at Hg(II) concentrations (mM)2 of:

 

0.5251020304050

Sensitivity to Hg(II) (S/R)3

PCR Amplification
with

merA specific primers

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

760C

control site

Moritella

+

 

 

 

 

 

 

 

S

-

 

 

 

 

Psychrophiles

760D

"

Psychrobacter

+

 

 

 

 

 

 

 

S

-

 

 

 

4°C

761F

"

Photobacterium

+

 

 

 

 

 

 

 

S

-

 

 

 

 

762G

"

Shewanella

+

 

 

 

 

 

 

 

S

-

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

763D

control site

Psychrobacter

+

+

 

 

 

 

 

 

S

-

 

 

 

 

EPR13

plume

Rhizobium

+

+

 

 

 

 

 

 

S

-

 

 

 

 

EPR11

vent

Halomonas

+

+

+

+

+

+

 

 

R

N. D.4

 

 

Control Site

Mesophiles

EPR1

plume

Pseudoalteromonas

+

+

+

+

+

+

+

 

R

-

 

 

Plume

28°C

EPR2

"

Pseudoalteromonas

+

+

+

+

+

+

+

 

R

-

 

 

Vent

 

EPR3

"

Pseudoalteromonas

+

+

+

+

+

+

+

 

R

Yes

 

 

 

 

EPR15

"

Marinobacter

+

+

+

+

+

+

+

 

R

N. D.

 

 

 

 

EPR12

"

Pseudomonas

+

+

+

+

+

+

+

+

R

Yes

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

EPR9

vent

Bacillus

+

+

 

 

 

 

 

 

S

-

 

 

 

Moderate

EPR5

plume

Alcanivorax

+

+

+

 

 

 

 

 

S

-

 

 

 

Thermophiles

EPR6

vent

Alcanivorax

+

+

+

+

+

+